Table 1.

Antibodies and reagents

Name	Company, product number, host	Dilution
FLAG M2	Sigma F3165, mouse	1:1,000
Rap2	BD Biosciences 610215, mouse	1:1,000
Tubulin	Li-Cor 926-42211, rabbit	1:2,500
GM130	BD Biosciences 610822, mouse	1:100
CD63	Gift from Andrew Peden (University of Sheffield, Sheffield, UK), mouse	1:100
EEA1	Gift from Andrew Peden, rabbit	1:100
Syntaxin13	Prekeris Lab (Prekeris et al., 1998), rabbit	1:100
Ubiquitin P4D1	Santa Cruz sc-8017, mouse	1:200
GFP	Invitrogen A-11122, rabbit	1:100
GAPDH	CST 5174, rabbit	1:1,000
EGFR	CST 2232, rabbit	1:1,000
Myc 9E10	Santa Cruz sc-40, mouse	1:1,000
HA	Santa Cruz sc-7392, mouse	1:1,000
FLAG-HRP conjugate	CST 86861, rabbit	1:1,000
AffiniPure goat anti-mouse IgG, light chain specific	Jackson ImmunoResearch 211-032-171	1:1,000
IRDye 680RD anti-mouse secondary	Li-Cor 926-68072	1:2,500
IRDye 800CW anti-rabbit secondary	Li-Cor 926-32213	1:2,500
Alexa Fluor 568 phalloidin	Thermo Fisher Scientific a12380	1:40
Alexa Fluor 488 anti-mouse secondary	Jackson ImmunoResearch 715-545-150	1:100
Alexa Fluor 594 anti-mouse secondary	Jackson ImmunoResearch 715-585-150	1:100
Alexa Fluor 594 anti-rabbit secondary	Jackson ImmunoResearch 711-585-152	1:100
Hoechst 33342	AnaSpec AS-83218	1:2,000
jetPRIME transfection reagent	Polyplus 114-xx	N/A
DharmaFECT Duo transfection reagent	Horizon discovery T-2010-xx	N/A
Lipofectamine 2000 transfection reagent	Invitrogen 11668027	N/A
tracrRNA	Horizon discovery U-002005-xx	N/A
Bafilomycin A1	Selleckchem S1413	200 nM
MLN4924	Selleckchem S7109	300 nM
Protein G Sepharose 4 Fast Flow	Cytiva 17-0618-01	50%
Bio-Rad Protein Assay Dye Reagent (Bradford method)	Bio-Rad 5000006	1:5
Pierce Silver Stain Kit	Thermo Fisher Scientific 24612	N/A
Intercept TBS Blocking Buffer	Li-Cor 927-60001	1:3
CytoPhos Phosphate Assay	Cytoskeleton BK054	N/A