FIGURE 1.

Volumetric Tissue Exploration and Analysis (VTEA) basic workflow. VTEA is a user-friendly platform that allows interactive exploration of image volume (1) where image processing, segmentation, analysis and exploration could occur in a single workspace (2). In the analytical plot, each dot represents a cell with various features. The simplest analysis is in the form of a 2D scatter plot displaying features on the x and y axis (3). Gates can be drawn to chose and quantify a specific population of cells that can be directly visualized in the image volume (4). Conversely, regions of interest can be drawn in the image (5) to locate cells of interest in the scatter plot (6). This process allows for an explorative interplay between the image and the analytical space. Red arrowhead shows different tabs available in the workspace. Figure adapted and used with permission from Winfree et al. (2017b).