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Gas production of the anaerobic cultures was recorded at 0, 12, 24, 36, and 48 h post inoculation (hpi), and the fermentation culture was collected at 24 and 48 hpi. Supernatants of the culture were separated by centrifugation for detection of pH and ammonia nitrogen levels. Gas production, NH3-N, and pH value during 48 h in vitro fecal fermentation with POP. (A) Gas production. (B) NH3-N. **P < 0.01 and ##P < 0.01 vs different groups, respectively. (C) pH value.
