Figure 2.

Diagrams of critical steps for EV isolation from Arabidopsis. (A) Diagram of where A. thaliana leaves should be cut to isolate the leaf blade and completely remove the petiole. (B) Diagram of how infiltrated leaves should be arranged on the tape strip. All leaves are oriented in the same way such that the leaf tips are at the top of the syringe and the base of the leaf blade is at the bottom of the syringe. The green arrow indicates the direction of the leaves on the tape. (C) Flowchart of how the taped leaves should be wrapped around the syringe, taped at the top, and the placed into 50 mL Falcon tubes for centrifugation.