Figure 2. Effect of Fusobacterium nucleatum on MMP9 expression in vitro. A549 cells were treated with 1.0×107, 5.0×107, or 1.0×108 CFU/ml F. nucleatum for 24 h. A: MMP9 mRNA expression was determined by real-time polymerase chain reaction. The data are presented as the mean±standard error of the mean; n=3. *Significantly different at p<0.05. The conditioned medium was analyzed by western blotting for MMP9 expression (B) and by gelatin zymography for MMP9 activity (C). Mock-treated samples were used as a control.
