Figure 2. Sorafenib, bufalin, or their combination treatment affected cell apoptosis in NCI-H292 cells. Cells (1×105 cells/well) were subjected to 15 μM sorafenib, 90 nM bufalin, or combination of 15 μM sorafenib with 90 nM bufalin for 48 h. Cells were treated with Annexin V/PI solution to determine the percentage of apoptosis, including earlier and late apoptotic cell death, by flow cytometric assays (A). The percentage of cell apoptosis was calculated (B). Data represent mean±SD. *p<0.05, **p<0.01, or ***p<0.001, a statistically significant difference between the two groups.
