Figure 3. Sorafenib, bufalin, or sorafenib combined with bufalin affected DNA condensation in NCI-H292 cells. Cells (1×105 cells/well) were subjected to 15 μM sorafenib, 90 nM bufalin, or the combination of 15 μM sorafenib with 90 nM bufalin for 48 h. After treatment, cells were stained with DAPI solution and then observed and photographed under a fluorescence microscope at 100× (A). The relative intensity of DAPI fluorescence in the nucleus was calculated (B). Data represent mean±SD. *p<0.05, **p<0.01, or ***p<0.001, a statistically significant difference between the two groups.
