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. 2021 Dec 27;221(2):e202109152. doi: 10.1083/jcb.202109152

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© 2021 Opalko et al.

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Figure 1. — Arf6 regulates cell size through the Cdr2-Wee1 pathway. (A) Schematic of the Cdr2-Wee1 pathway. (B) SGA workflow. (C) Example of SGA growth screening. Strains were spotted in quadruplicate. Yellow box shows slow growth; red box shows no growth. (D) Venn diagram of SGA hits. (E) Serial dilution assay. (F and G) Surface area (F) and volume (G) of dividing cells from the indicated strains. n ≥ 70 cells per strain; ns, P ≥ 0.05; ***, P ≤ 0.001 by one-way ANOVA followed by Tukey’s multiple comparison test. Graphs show mean ± SD. (H) Western blot of whole-cell extracts for endogenous Wee1. Asterisks indicate background bands; Cdc2 is loading control. Source data are available for this figure: SourceData F1.