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. Author manuscript; available in PMC: 2022 Mar 18.
Published in final edited form as: Matrix Biol. 2020 Jun 17;93:79–94. doi: 10.1016/j.matbio.2020.06.002

Fig. 2. FP-HSP47 colocalizes with procollagen in the ER but does not colocalize with the cis-Golgi marker GM130.

Fig. 2.

(A,B) MC3T3 cells transfected with Cherry-HSP47, Venus-proα1(I) (A) or Venus-proα2(I) (B), and marker of ER lumen (ssCFP-KDEL). (C,D) MC3T3 cells transfected with Cherry-HSP47 (C) or TagBFP2-HSP47 (D), Venus-proα1(I) (C) or GFP-proα2(I) (D) and marker of cis-Golgi CFP-GM130 (C) or Cherry-GM130 (D). Switching of the fluorescent tag colors in different images illustrates that each of these and subsequent experiments were performed with different combinations of fluorescent tag colors, to eliminate potential tag-specific effects. Arrows mark Golgi structures containing procollagen. All images are Airyscan (A,B) or confocal (C,D) single slices; scale bars = 10 μm (full cell) and 1 μm (zoomed areas); N≥20 (≥4 experiments with different FP combinations) for each panel.