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. Author manuscript; available in PMC: 2022 Mar 18.
Published in final edited form as: Matrix Biol. 2020 Jun 17;93:79–94. doi: 10.1016/j.matbio.2020.06.002

Fig. 4, Movies 3,4. Dynamics of vesicle-like FP-HSP47 structures resembles stochastic fluctuations of the reticular ER network but not rapid directional movement of FP-procollagen transport vesicles.

Fig. 4,

(A, Movie 3) Confocal single slice frame and 2s/frame time-lapse video of a cell transfected with GFP-proα1(I) and Apple-HSP47. (B, Movie 4) Airyscan single slice frame and 1 s/frame video of a cell transfected with Venus-proα2(I) and Cherry-HSP47. Some of procollagen transport vesicles identified by rapid directional movement are outlined by white circles in the time-lapse videos and also marked by arrows in the still frames. These transport intermediates contain procollagen but not HSP47. Stochastically moving vesicle-like structures containing procollagen and HSP47 are outlined by magenta circles in the videos and also marked by arrowheads in the still frames. Scale bars = 10 μm (whole cell) and = 1 μm (zoom); N>70 (≥14 experiments with different FP combinations) for each panel.