Fig. 6.

Elevated miR-140-3p restrains proliferation and facilitates apoptosis of RASFs and this effect can be reversed by overexpressed SGK1. A Expression of miR-140-3p and SGK1 in RASFs was detected by RT-qPCR; B Protein expression of SGK1 in RASFs was measured using Western blot analysis; C Contents of TNF-α and IL-1β in RASFs were evaluated by ELISA; D SOD activity in RASFs was measured by ELISA; E MDA content in RASFs was measured by ELISA; F MTT assay was applied to assess the proliferation of RASFs; G Apoptosis of RASFs was detected by flow cytometry; H Migration ability of RASFs was evaluated by Transwell assay; N = 3; *P < 0.05 vs the mimic-NC group, ^#P < 0.05 vs the miR-140-3p mimic group; the data were expressed as mean ± standard deviation, one-way ANOVA was used for comparisons among multiple groups and Tukey’s post hoc test was used for pairwise comparisons after ANOVA