Fig. 2.

Transient gene expression in Citrus sinensis protoplasts. The average number of EGFP positive cells in ‘N7-3’ protoplast cultures were greater in those treated with Lipofectamine LTX + the Arg9 CCP compared to those treated with Lipofectamine LTX only (A). Error bars represent standard error. Brightfield (B) and fluorescent (C) images showed that some of the protoplasts were EGFP positive 72 h after transfection. Confocal image of a protoplast cell indicating that the Arg9 CPP conjugated with TAMRA penetrated the cell membrane but not the nuclear membrane. There was no colocalization between the nucleus stained with DAPI (D) and the TAMARA signal (E) indicated by the merge (F). Scale bar indicates 100 µM in length