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. 2022 Mar 18;23:16. doi: 10.1186/s12868-022-00698-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Paeonol suppresses the pyroptosis and inflammatory responses in BV-2 cells. A The mRNA expression of caspase1 and NLRP3 in BV-2 cells was detected by qRT-PCR. ***P < 0.001 vs. the control group. B The western blot assay images (20 μg/lane) for the levels of ASC, NLRP3, active caspase 1, and N-GSDMD in BV-2 cells. C The protein level of ASC in BV-2 cells was measured by western blot assay. D The protein level of NLRP3 in BV-2 cells was measured by western blot assay. E The protein level of active caspase 1 in BV-2 cells was measured by western blot assay. F The protein level of N-GSDMD in BV-2 cells was measured by western blot assay. G The level of IL-1β i in BV-2 cells was measured by ELISA. H The level of IL-18 in BV-2 cells was measured by ELISA. I The level of TNF-α in BV-2 cells was measured by ELISA. ***P < 0.001 vs. the control group. ^##P < 0.01, ^###P < 0.001 vs. the LPS/ATP group