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. 2022 Mar 17;17(1):556–565. doi: 10.1515/med-2022-0429

Figure 2.

Figure 2

Effect of miR-22-5p on SSCs. Human SSCs were transfected with the miR-22-5p mimic or miR-22-5p inhibitor, respectively. (a) The miR-22-5p overexpression and interference efficiency were detected by QRT-PCR. (b and c) Cell proliferation was measured by EdU staining. (d and e) Cell apoptosis was analyzed by Annexin V/PI staining. (f and g) The expression of SSC markers (GDNF and DAZL) and apoptosis-related proteins (Caspase-3, Bax, and Bcl-2) were detected by western blot. *p < 0.05, **p < 0.01 vs mimic NC; #p < 0.05, ##p < 0.01 vs inhibitor NC.