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Primer sequences used in miRNA reverse transcription and PCR
| Name | Primer (5′-3′) |
|---|---|
| Reverse transcription primers | |
| miR-22-5p | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCA |
| CTGGATACGACTAAAGC | |
| PCR primers | |
| miR-22-5p | F: GAGCTGCACTGACCAGTAGG |
| R: GTGCTGGCAGATGGATCACT | |
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT | |
| EZH2 | F: CGGGGTACCGAGTCATACTTGTGAAG |
| R: GCACTCGAGCCTGTTTTTGTTTGATG | |
| GAPDH | F: GCACCGTCAAGGCTGAGAAC |
| R: TGGTGAAGACGCCAGTGGA | |
