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. 2022 Mar 17;17(1):536–549. doi: 10.1515/med-2022-0434

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© 2022 Wei Wei et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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PDZD8 overexpression rescues the inhibitory effect of circ_0020123 knockdown on LUAD cell proliferation and migration. (a) The mRNA expression and protein levels of PDZD8 were increased after transfection of pcDNA3.1/PDZD8 into LUAD cells. **p < 0.01, ***p < 0.001 vs pcDNA3.1 group. (b and c) Colony formation assays and EdU assays revealed that PDZD8 overexpression rescued the inhibitory effect of circ_0020123 silencing on LUAD cell proliferation. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (d) Transwell assays showed that the suppressive effect of circ_0020123 depletions on LUAD cell migration was reversed by PDZD8 overexpression. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (e) Wound healing assays were performed to detect the migration of LUAD cells transfected with sh-NC, sh-circ_0020123#1, or sh-circ_0020123#1 + PDZD8. ***p < 0.001 vs sh-NC group or sh-circ_0020123#1 group. (f) The relationship of circ_0020123, miR-1283, and PDZD8 as well as their roles in LUAD cells has been illustrated by a schematic diagram. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.

PDZD8 overexpression rescues the inhibitory effect of circ_0020123 knockdown on LUAD cell proliferation and migration. (a) The mRNA expression and protein levels of PDZD8 were increased after transfection of pcDNA3.1/PDZD8 into LUAD cells. **p < 0.01, ***p < 0.001 vs pcDNA3.1 group. (b and c) Colony formation assays and EdU assays revealed that PDZD8 overexpression rescued the inhibitory effect of circ_0020123 silencing on LUAD cell proliferation. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (d) Transwell assays showed that the suppressive effect of circ_0020123 depletions on LUAD cell migration was reversed by PDZD8 overexpression. ***p < 0.001 vs sh-NC group, **p < 0.01 vs sh-circ_0020123#1 group. (e) Wound healing assays were performed to detect the migration of LUAD cells transfected with sh-NC, sh-circ_0020123#1, or sh-circ_0020123#1 + PDZD8. ***p < 0.001 vs sh-NC group or sh-circ_0020123#1 group. (f) The relationship of circ_0020123, miR-1283, and PDZD8 as well as their roles in LUAD cells has been illustrated by a schematic diagram. The data are presented as the mean value ± standard deviation. One-way analysis of variance followed by Tukey’s post hoc analysis was used to compare differences among groups.