Table 3.

Oligonucleotides

Primer name	Primer sequence	Purpose
Acr3.Xho1.For	5′-GAT​Cct​cga​gGA​ATT​TAG​CAG​CTA​CTT​TTG​GTC-3′	ACR3 cloning
Acr3.Not1.Rev	5′-GAT​Cgc​ggc​cgc​ATT​TCT​ATT​GTT​CCA​TAT​ATA​ATA​TGG​TTT​AAG​G-3′	ACR3 cloning
Acr3.XbaI.For	5′-GAT​Ctc​tag​aAT​GTC​AGA​AGA​TCA​AAA​AAG-3′	ACR3 cloning
Acr3-C169S.sense	5′-GAG​GAG​ACA​ATG​ATC​TCA​GCG​TCG​TGC​TTG​TTA​TT-3′	Acr3-C169S
Acr3-169S.antisense	5′-AAT​AAC​AAG​CAC​GAC​GCT​GAG​ATC​ATT​GTC​TCC​TC-3′	Acr3-C169S
Acr3-C316S.sense	5′-GAG​TGA​TAC​ACA​AAG​AGA​AAG​TAG​CTG​TGA​CCA​AGA​ACT-3′	Acr3-C316S
Acr3-C316S.antisense	5′-AGT​TCT​TGG​TCA​CAG​CTA​CTT​TCT​CTT​TGT​GTA​TCA​CTC-3′	Acr3-C316S
Acr3-C318S.sense	5′-GTG​ATA​CAC​AAA​GAG​AAT​GTA​GCA​GTG​ACC​AAG​AAC​TAC​TTT​TAA​AG-3′	Acr3-C318S
Acr3-C318S.antisense	5′-CTT​TAA​AAG​TAG​TTC​TTG​GTC​ACT​GCT​ACA​TTC​TCT​TTG​TGT​ATC​AC-3′	Acr3-C318S
Acr3-C333S.sense	5′-GTC​TGG​GGA​AGA​AAG​TCT​AGT​GAA​GCT​AGC​TTT​TCT​A-3′	Acr3-C333S
Acr3-C333S.antisense	5′-TAG​AAA​AGC​TAG​CTT​CAC​TAG​ACT​TTC​TTC​CCC​AGA​C-3′	Acr3-C333S
Acr3-del307-334.sense	5′-ACC​TTC​GCA​TTA​ATG​AGG​TAC​TTA​TCA​GCT​AGC​TTT​TCT​ATT​ACG-3′	Acr3 loop deletion
Acr3-del307-334.antisense	5′CGT​AAT​AGA​AAA​GCT​AGC​TGA​TAA​GTA​CCT​CAT​TAA​TGC​GAA​GGT-3′	Acr3 loop deletion
Acr3.start.Xba1.for	5′-GAT​Ctc​tag​aAT​GTC​AGA​AGA​TCA​AAA​AAG​TGA-3′	Acr3 BIFC
Acr3.end.BspE1.Rev	5′-GAT​Ctc​cgg​aAT​TTC​TAT​TGT​TCC​ATA​TAT​AAT​A-3′	Acr3 BIFC
HPH.Acr3del.For	5′-TGA​ATG​CTC​TCG​TTG​TAA​TTC​AAG​AGA​ACC​CAA​CCA​ACA​AAT​CAT​CAG​GTT​AGT​AGA​ATA​TTT​AGC​TTG​CCT​TGT​CCC​CG-3′	ACR3 genomic deletion
HPH.Acr3del.Rev	5′-GAG​TTA​TAA​GTG​GTA​TTA​TTC​ATT​GGT​GCC​CAA​GTA​ATT​TTT​TGG​TGA​GTC​AAC​AAT​GAT​TAT​TCC​TTT​GCC​CTC​GG-3′	ACR3 genomic deletion
Hog1-C38S.sense	5′-GGG​GCA​TTT​GGG​TTG​GTT​AGC​TCA​GCC​ACG-3′	Hog1-C38S
Hog1-C38S.antisense	5′-CGT​GGC​TGA​GCT​AAC​CAA​CCC​AAA​TGC​CCC-3′	Hog1-C38S
Hog1-C156S.sense	5′-GCA​ACA​TTC​TGA​TTA​ATG​AAA​ACA​GTG​ATT​TGA​AGA​TTT​GCG​ATT​TC-3′	Hog1-C156S
Hog1-C156S.antisense	5′-GAA​ATC​GCA​AAT​CTT​CAA​ATC​ACT​GTT​TTC​ATT​AAT​CAG​AAT​GTT​GC-3′	Hog1-C156S
Hog1-161S.sense	5′-TCT​GAT​TAA​TGA​AAA​CTG​TGA​TTT​GAA​GAT​TAG​CGA​TTT​CGG​TCT​AGC-3′	Hog1-C161S
Hog1-161S.antisense	5′-GCT​AGA​CCG​AAA​TCG​CTA​ATC​TTC​AAA​TCA​CAG​TTT​TCA​TTA​ATC​AGA-3′	Hog1-C161S
Hog1-C205S.sense	5′-GAC​ATT​TGG​TCC​GCT​GGT​AGT​ATT​TTT​GCC​GAA​ATG​A-3′	Hog1-C205S
Hog1-C205S.antisense	5′-TCA​TTT​CGG​CAA​AAA​TAC​TAC​CAG​CGG​ACC​AAA​TGT​C-3′	Hog1-C205S
Rgc2.start.BspE1.for	5′-GAT​Ctc​cgg​aTC​TGA​TTA​CTT​TAG​TTC​C-3′	Rgc2 BIFC
Rgc2.end.XhoI.rev	5′-GAT​Cct​cga​gTT​ACT​GTT​GGA​ACT​CAT​TAG​CGT​ACT-3′	Rgc2 BIFC
Ura to His.For	5′-GGC​ATC​AGA​GCA​GAT​TGT​ACT​GAG​AGT​GCA​CCA​CGC​TTT​CGG​ATC​CCC​GGG​TTA​ATT​AA-3′	RGC2 with HIS3 marker
Ura to His.Rev	5′-TTT​AGT​ATA​CAT​GCA​TTT​ACT​TAT​AAT​ACA​GTT​TTT​TAG​GAA​TTC​GAG​CTC​GTT​TAA​AC-3′	RGC2 with HIS3 marker
pMet25.Sac1.For	5′-GAT​Cga​gct​cAG​CTC​CGG​ATG​CAA​GGG​TTC-3′	MTQ2 cloning
pMet25.start.Mtq2.Rev	5′-CTT​TAT​CGT​AAT​CGC​ATT​TTA​CAT​AAG​GGG​TCG​GTA​GCA​TTA​GTT​TGA​GCC​CAC​TAG​TTC-3′	MTQ2 cloning
pMet25.start.Mtq2.For	5′-TAC​CCC​CAT​CCA​TAC​TCT​AGA​ACT​AGT​GGG​CTC​AAA​CTA​ATG​CTA​CCG​ACC​CCT​TAT​GT-3′	MTQ2 cloning
Mtq2.His6X.XhoI.Rev	5′-GAT​Cct​cga​gCT​AAT​GAT​GAT​GAT​GAT​GAT​GAC​CCC​TTG​TAA​AGC​TGT​ACA​CAC​TGA-3′	MTQ2 cloning

Restriction sites are in lower case.