HFD-induced obesity suppresses immune cell trafficking to the tumor. C57BL/6 mice were fed an HFD or SFD for 9–12 wk, and B16-F10 tumors were injected. CD45.2-PE was injected i.v. 10 min before culling. This experiment was performed twice. (A) Exemplary image of flow cytometric analysis of tumors from SFD- and HFD-fed mice. Boxes indicate CD45.2iv− tumor-resident population. (B) Proportion of tumor-resident leukocytes (CD45.2iv−) and CD8+ T cells in B16-F10 tumors. (C) Number of tumor-circulating, tumor-resident, and total CD8+ T cells in B16-F10 tumors. (D and E) Flow cytometric analysis of CXCR3 and CD49d expression on CD8+ T cells in B16.F10 tumors. (F) qPCR on total B16-F10 tumors from SFD-fed (n = 8) and HFD-fed (n = 11) mice from two pooled experiments. Data are shown as individual mice (dots) and mean ± SEM. Significance was calculated using an unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.