Chemokine and chemokine receptor expression in tumors of HFD-fed mice.
(A) Flow cytometric analysis of CXCR3 expression by CD8+ T cells on day 17 after tumor injection in MC38 tumors of SFD-fed (n = 7) or HFD-fed (n = 8) mice. Dots represent individual mice. P = 0.08 using unpaired Student’s t test. (B) SFD and HFD mice were injected with B16-F10 tumors, and HFD mice were injected s.c. with rmIFN-γ on days 5, 8, 11, and 13. Experiment was performed twice (n = 4–7 per group). Relative expression of Cxcl9 and Cxcl10 from total tumors on day 14 after tumor injection. Data are shown as individual mice and mean ± SEM; one-way ANOVA. ***, P < 0.001.