Figure S2.

CD8 T cell activation in dLNs is not impaired in obese mice. (A and B) C57BL/6 mice were fed an SFD or HFD, and MC38 (A) or B16-F10 (B) tumor cells were injected s.c. Results depict flow cytometric analysis of CD8⁺ T cells in dLNs of tumor-bearing mice. Data are shown as individual mice and mean ± SEM; unpaired Student’s t test. **, P < 0.01 for three independent experiments for each tumor model. (C and D) CD8 T cells isolated from SFD-fed (n = 3; pooled) or HFD-fed (n = 3; pooled) mice were activated with anti-CD3/anti-CD28 and analyzed by Seahorse flux assay (C) or flow cytometry (D). Oxphos, oxidative phosphorylation. (E) CD8 T cells isolated from dLNs or spleens from SFD-fed (black; n = 3 technical replicates from 7 pooled mice) or HFD-fed (red; n = 3 technical replicates from 7 pooled mice) mice were activated with anti-CD3/anti-CD28 (+) or unstimulated (−) and analyzed by flow cytometry. Representative data from at least two independent experiments.