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. 2022 Jan 19;219(3):e20211001. doi: 10.1084/jem.20211001

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© 2022 Liu et al.

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Figure 7. — Changes in transcriptional profiling and up-regulation of ERK1/2 signaling are associated with the effect of Ang II on ILC2s. (A–E) Lung ILC2s were cultured with IL-33 or IL-33 plus Ang II for 6 h, and transcriptional profiling was evaluated by SMART-seq2. (A) Principal components analysis (PCA). (B) Kyoto Encyclopedia of Genes and Genomes analysis. (C) Heatmap of expression of selected genes related to the cell cycle. (D) Heatmap of known ILC2 regulators. (E) Heatmap of selected genes of Ras and MAPK signaling pathways. (F and G) Levels of Erk1/2 phosphorylation (p-ERK1/2) in lung ILC2s from AT1a^+/+ and AT1a^−/− mice were determined by flow cytometry after stimulation with Ang II or PBS (F), or PMA or DMSO (G), for 15 min. (H) Amounts of IL-5 and IL-13 in culture supernatants of ILC2s from AT1a^+/+ and AT1a^−/− mice were determined by ELISA, after treatment with PMA and/or U0126 for 72 h. (I) Mean fluorescence intensity (MFI) of p-STAT5 in lung ILC2s from AT1a^+/+ and AT1a^−/− mice after stimulation with PBS or Ang II for 15 min. Data are representative of two independent experiments. Graphical data show mean ± SEM; unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.