Video 4.
Fluorescent time-lapse recording of cultured MicroDREADDDq microglia cells responding to 1 µM C21 DREADD agonist. The cells were loaded with Cal590 AM fluorogenic calcium sensitive dye (red) and the cell membrane was stained with the FluoVolt membrane labeling dye (green). The video was recorded at 0.25 frame/s in a 150 × 107-µm field of view. Scale bar, 10 µm. Note decreased process motility already after the first C21 treatment and markedly decreased calcium responses to repeated C21 stimulations.