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Representative in vivo two-photon imaging videos recorded by the resonant scanner showing reduced hypercapnia-induced vasodilation in P2Y12R KO (CX3CR1GFP/+ × P2Y12R KO) mice compared with control (CX3CR1GFP/+) mice. Blood vessels were visualized by administration of Rhodamine B-dextran. Hypercapnia was induced at 60 s and maintained until 180 s with a 60-s-long posthypercapnic period. Identical fields of view are shown in Fig. 5 j.
