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. 2022 Feb 26;219(3):e20211403. doi: 10.1084/jem.20211403

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© 2022 Bigley et al.

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Figure S5. — Neonatal MRV infection results in transiently decreased CD4⁺ T cells in the periphery. (A and B) BALB/c mice were mock or MRV infected on D0 and harvested at various time points for flow cytometry of the spleen, shown as absolute number (#) of cells per spleen (A), or percentage Foxp3⁺ cells per total CD4⁺ cells (B). n = 3–6 for each time point from two independent infections. Statistical significance was evaluated used multiple comparison Student’s t test for flow cytometry. (C–E) D0 mock- or MRV-infected thymus from mice 3, 5, or 7 dpi were evaluated by flow cytometry. CD19⁻NKp46⁻TCRγδ⁻CD25⁻ (Lin⁻) cells were gated on CD5, and TCRβ surface expression and absolute number (#) were evaluated for CD5^hiTCRβ^hi or CD5^loTCRβ^lo (C) and CD4 SP (CD4⁺), DP (CD4⁺CD8⁺), or CD4⁺Foxp3⁺ (D). Splenocytes were evaluated at the designated time points and evaluated for absolute number (#) of total CD4⁺ cells or CD4⁺Foxp3⁺ Tregs (E). n = 6 for all except mock 3 dpi (n = 4), each from two to three separate infections. Statistical significance was calculated using multiple comparison Student’s t test. **, P < 0.01; ***, P < 0.001.