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. 2022 Mar 3;18(3):e1010091. doi: 10.1371/journal.pgen.1010091

Fig 2. Overexpression of FLP-18 causes locomotion defects.

Fig 2

(A) Behavioral tracking of wild type, flp-18(gk3063) mutants, and transgenic animals that overexpress FLP-18 (FLP-18(+++)). Representative images of each genotype (upper panel) and locomotion tracks recorded from multiple animals freely moving on a plate for 5 min (lower panel). Scale bars represent 200 μm (top) and 5 mm (bottom). (B) Schematic illustrating quantified behaviors. (C-F) Quantification of locomotion behavior. (C) Speed of foraging head movements. (D) locomotion velocity. (E) Body bend curvature. (F) Spontaneous reversal frequency. (G) Kymographs showing body bending along the body over 30 s of locomotion. Color map represents degrees of bending, positive values represent dorsal bends and negative values represent ventral bends. Graphs represent mean ± SEM, significance was calculated using ANOVA with Dunnett’s multiple comparison test (P<0.005 = **, P<0.0001 = ****). Sample sizes: (C), wild type (n = 38), flp-18 (n = 28), FLP-18(+++) (n = 31). (D-F) wild type, flp-18, and FLP-18(+++) (n = 16 experiments per genotype, 20 animals per experiment).