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. 2022 Mar 18;17(3):e0261485. doi: 10.1371/journal.pone.0261485

Table 1. Summary of the applied anhydrobiosis protocols.

In the case of A and B protocols, each plate contained five additional specimens for tuns’ photography under stereomicroscope and tuns’ ultrastructure analysis under transmission electron microscopy.

Protocol Quantitative details Mode of dehydration Substratum
A. 10 repeats, each for 20 individuals preconditioning agar layer
(20 specimens per plate)
B. 5 repeats, each for 50 individuals slow dehydration filter paper
(50 specimens per plate)
C1. 5 repeats, each for 50 individuals environmental drying moist fine sand
(50 specimens per plate)
C2. 5 repeats, each for 50 individuals environmental drying sediment from the pond
(50 specimens per plate)