Table 1. Summary of the applied anhydrobiosis protocols.
In the case of A and B protocols, each plate contained five additional specimens for tuns’ photography under stereomicroscope and tuns’ ultrastructure analysis under transmission electron microscopy.
Protocol | Quantitative details | Mode of dehydration | Substratum |
---|---|---|---|
A. | 10 repeats, each for 20 individuals | preconditioning | agar layer |
(20 specimens per plate) | |||
B. | 5 repeats, each for 50 individuals | slow dehydration | filter paper |
(50 specimens per plate) | |||
C1. | 5 repeats, each for 50 individuals | environmental drying | moist fine sand |
(50 specimens per plate) | |||
C2. | 5 repeats, each for 50 individuals | environmental drying | sediment from the pond |
(50 specimens per plate) |