FIG. 2.

Physical map of the insert of plasmid pAC2AL and subcloning strategy. Thick lines represent cloned DNA, and thin lines correspond to vector sequences. Plasmids whose names begins with pAC are pACYC184 derivatives, while those whose name begins with pBC are pBC-SK derivatives. Production of metallo-β-lactamase activity (β-lact.) was assayed with crude extracts as described in Materials and Methods. B, BamHI; C, ClaI; H, HindIII; Sa, SalI; Sm, SmaI; S/B, Sau3AI/BamHI junction; V, EcoRV; X, XhoI.