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. 2022 Mar 17;11(1):164–174. doi: 10.1080/21623945.2022.2044601

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Analysis of adipogenic markers in the course of differentiation. (a) mRNA expression of adipogenic genes measured by RT-qPCR. A representative result of n = 3 different donors is shown. Values are presented as mean ±SEM of three technical replicates. Statistical analysis was done using one-way ANOVA with Dunnett's Multiple Comparison Test. (b) Western blot analysis of adipogenic markers corresponding to (a). Left panel: A representative result of n = 3 different donors is shown. β-Actin served as loading control. Right panel: Densitometric analysis. Values are presented as mean ± SEM of three measurements. Statistical analysis was done using one-way ANOVA with Dunnett's Multiple Comparison Test. (c) Adiponectin ELISA of cell culture supernatants corresponding to (a) and (b). n. d.: not determined.