Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Xrp1 | GenBank | FLYBASE:FBgn0261113 | |
| Gene (Drosophila melanogaster) | RpS12 | GenBank | FLYBASE: FBgn0286213 | |
| Gene (Drosophila melanogaster) | RpS18 | GenBank | FLYBASE:FBgn0010411 | |
| Gene (Drosophila melanogaster) | RpL27A | GenBank | FLYBASE:FBgn0285948 | |
| Gene (Drosophila melanogaster) | RpS3 | GenBank | FLYBASE:FBgn0002622 | |
| Gene (Drosophila melanogaster) | RpS17 | GenBank | FLYBASE:FBgn0005533 | |
| Gene (Drosophila melanogaster) | RpL14 | GenBank | FLYBASE:FBgn0017579 | |
| Gene (Drosophila melanogaster) | RpL19 | GenBank | FLYBASE:FBgn0285950 | |
| Gene (Drosophila melanogaster) | RpL36 | GenBank | FLYBASE:FBgn0002579 | |
| Gene (Drosophila melanogaster) | TAF1B | GenBank | FLYBASE:FBgn0037792 | |
| Gene (Drosophila melanogaster) | PPP1R15 | GenBank | FLYBASE:FBgn0034948 | |
| Gene (Drosophila melanogaster) | PERK | GenBank | FLYBASE:FBgn0037327 | |
| Gene (Drosophila melanogaster) | Gcn2 | GenBank | FLYBASE:FBgn0019990 | |
| Gene (Drosophila melanogaster) | Irbp18 | GenBank | FLYBASE:FBgn0036126 | |
| Gene (Drosophila melanogaster) | eIF4G | GenBank | FLYBASE:FBgn0023213 | |
| Gene (Drosophila melanogaster) | eEF2 | GenBank | FLYBASE:FBgn0000559 | |
| Gene (Drosophila melanogaster) | eIF6 | GenBank | FLYBASE:FBgn0034915 | |
| Gene (Drosophila melanogaster) | copia | GenBank | FLYBASE:FBgn0013437 | |
| Genetic reagent (D. melanogaster) | eEF1α1 | GenBank | FLYBASE:FBgn0284245 | |
| Genetic reagent (D. melanogaster) | eIF5A | GenBank | FLYBASE:FBgn0285952 | |
| Genetic reagent (D. melanogaster) | Xrp1HA | Blanco et al., 2020 | Strain maintained in Dr. Nicholas Baker’s lab. | |
| Genetic reagent (D. melanogaster) | Xrp1M2-73 allele | Lee et al., 2018 | FLYBASE:RRID:BDSC_81270 | Bloomington Drosophila Stock Center#81,270 |
| Genetic reagent (D. melanogaster) | RpS12 G97D allele | Tyler et al., 2007 | FLYBASE:FBal0193403 | Strain maintained in Dr. Nicholas Baker’s lab. |
| Genetic reagent (D. melanogaster) | UAS-dsRNAXrp1 | Perkins et al., 2015 | FLYBASE:RRID:BDSC_34521 | Bloomington Drosophila Stock Center#34,521 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAXrp1 | Dietzl et al., 2007 | FLYBASE:FBti0118620 | Vienna Drosophila Resource Center#v 107,860 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAirbp18 | Perkins et al., 2015 | FLYBASE:RRID:BDSC_33652 | Bloomington Drosophila Stock Center#33,652 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAw | Perkins et al., 2015 | FLYBASE:RRID:BDSC_33623 | Bloomington Drosophila Stock Center#33,623 |
| Genetic reagent (D. melanogaster) | arm-LacZ | Vincent et al., 1994 | FLYBASE:FBal0040819 | |
| Genetic reagent (D. melanogaster) | Ubi-GFP | Davis et al., 1995 | FLYBASE:FBal0047085 | |
| Genetic reagent (D. melanogaster) | M(2)56 F(mutating RpS18) | Laboratory of Y. Hiromi | FLYBASE:FBal0011916 | |
| Genetic reagent (D. melanogaster) | Df(1)R194 (deleting RpL36) | Duffy et al., 1996 | FLYBASE:FBab0024817 | |
| Genetic reagent (D. melanogaster) | P{RpL36+} | Tyler et al., 2007 | FLYBASE:FBal0193398 | |
| Genetic reagent (D. melanogaster) | M{RpL19+} | Baillon et al., 2018 | ||
| Genetic reagent (D. melanogaster) | Df(2 R)M60E (deleting RpL19) | Baillon et al., 2018 | FLYBASE:FBab0001997 | |
| Genetic reagent (D. melanogaster) | hs-FLP | Struhl and Basler, 1993 | FLYBASE:FBtp0001101 | |
| Genetic reagent (D. melanogaster) | P{GAL4-Act5C(FRT.CD2).P}S | Pignoni and Zipursky, 1997 | FLYBASE:FBti0012408 | Bloomington Drosophila Stock Center#51,308 |
| Genetic reagent (D. melanogaster) | P{neoFRT}42D | Xu and Rubin, 1993 | FLYBASE:FBti0141188 | Bloomington Drosophila Stock Center#1,802 |
| Genetic reagent (D. melanogaster) | P{neoFRT}80B | Xu and Rubin, 1993 | FLYBASE:FBti0002073 | Bloomington Drosophila Stock Center#1988 |
| Genetic reagent (D. melanogaster) | P{neoFRT}82B | This study | FLYBASE:FBti0002074 | Viable line derived from Bloomington Drosophila Stock Center lines BL5188 and BL30555 |
| Genetic reagent (D. melanogaster) | UAS-dsRNATAF1B | Perkins et al., 2015 | RRID:BDSC_61957 | Bloomington Drosophila Stock Center#61,957 |
| Genetic reagent (D. melanogaster) | UAS-dsRNATAF1B | Dietzl et al., 2007 | FLYBASE:FBti0118760 | Vienna Drosophila Resource Center#v105873 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAeIF6 | Dietzl et al., 2007 | FLYBASE:FBti0116845 | Vienna Drosophila Resource Center#v108094 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAeIF4G | Dietzl et al., 2007 | FLYBASE:FBti0095456 | Vienna Drosophila Resource Center#v17002 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAeIF5A | Dietzl et al., 2007 | FLYBASE:FBti0121478 | Vienna Drosophila Resource Center#v101513 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAeEF2 | Dietzl et al., 2007 | FLYBASE:FBti0117284 | Vienna Drosophila Resource Center#v107268 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAeEF1α1 | Dietzl et al., 2007 | FLYBASE:FBti0121842 | Vienna Drosophila Resource Center#v104502 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAPERK | Dietzl et al., 2007 | FLYBASE:FBti0141304 | Vienna Drosophila Resource Center# v110278 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAPERK | Dietzl et al., 2007 | FLYBASE:FBti0093363 | Vienna Drosophila Resource Center#v 16,427 |
| Genetic reagent (D. melanogaster) | UAS-dsRNAGcn2 | Dietzl et al., 2007 | FLYBASE:FBti0118018 | Vienna Drosophila Resource Center#v103976 |
| Genetic reagent (D. melanogaster) | RpS18 mutation M(2 R)56 f | Laboratory of Y. Hiromi | FLYBASE:FBal0284387 | |
| Genetic reagent (D. melanogaster) | RpS3 | Burke and Basler, 1996 | Flybase: FBgn0002622 | |
| Genetic reagent (D. melanogaster) | RpL27A Df(2 L)M24F11 | Marygold et al., 2007 | Flybase: FBab0001492 | |
| Genetic reagent (D. melanogaster) | RpS17 mutation M(3 L)67 C4 | Morata and Ripoll, 1975 | Flybase: FBal0011935 | |
| Genetic reagent (D. melanogaster) | en-Gal4 | Neufeld et al., 1998 | RRID:BDSC_6356 | |
| Genetic reagent (D. melanogaster) | UAS-S65T-GFP | FBrf0086268 | FBtp0001403 | |
| Genetic reagent (D. melanogaster) | P[GAL4-Act5C(FRT.CD2).P]S | FBrf0221941 | FBti0012408 | |
| Genetic reagent (D. melanogaster) | P[UAS-His-RFP]3 | FBrf0221941 | FBti0152909 | |
| Antibody | anti-active-Dcp1 (rabbit polyclonal) | Cell Signalling Technology | Cat #9,578RRID:AB_2721060 | (1:100) |
| Antibody | anti-XRP1(short)(rabbit polyclonal) | Francis et al., 2016 | (1:200) | |
| Antibody | antiphospho-RpS6(rabbit polyclonal) | Romero-Pozuelo et al., 2017 | (1:200) | |
| Antibody | anti-p62 (rabbit polyclonal) | Pircs et al., 2012 | (1:300) | |
| Antibody | anti-phospho-eIF2α (rabbit polyclonal) | Thermo Fisher Scientific | Cat #44–728 GRRID:AB_2533736 | (1:200) |
| Antibody | anti-phospho-eIF2α (D9G8)(rabbit monoclonal) | Cell Signaling Technology | Cat #D9G8#3398 RRID:AB_10829234 | (1:200) |
| Antibody | anti-HATag (mouse monoclonal) | Cell Signalling Technology | Cat #2,367RRID:AB_10691311 | (1:100) |
| Antibody | anti-beta galactosidase (mAb40-1a) (mouse monoclonal) | DSHB | RRID: AB_2314509 | (1:100) |
| Antibody | anti-Mouse IgG, Cy2(goat monoclonal) | Jackson Immunoreseach | Cat #115-225-166 RRID:AB_2338746 | (1:200) |
| Antibody | anti-Mouse IgG, Alexa Fluor 555(goat polyclonal) | Thermo Fischer Scientific | Cat #A28180 RRID:AB_2536164 | (1:200) |
| Antibody | anti-Mouse IgG, Alexa Fluor 647(Goat polyclonal) | Thermo Fischer Scientific | Cat #A-21235 RRID:AB_2535804 | (1:200) |
| Antibody | anti-Mouse IgG, Alexa Fluor 488(Goat polyclonal) | Thermo Fischer Scientific | Cat #A-11001RRID:AB_2534069 | (1:400) |
| Antibody | anti-Rabbit Cy3,(Goat polyclonal) | Thermo Fischer Scientific | Cat #A-21244 RRID:AB_2535812 | (1:200) |
| Antibody | anti-Rabbit IgG, Alexa Fluor 555(Goat polyclonal) | Thermo Fischer Scientific | Cat #A21429 RRID:AB_2535850 | (1:300) |
| Antibody | anti-Rabbit IgG, Alexa Fluor 647(Goat polyclonal) | Thermo Fischer Scientific | Cat #A-21244 RRID:AB_2535812 | (1:200) |
| Antibody | anti-Guinea Pig Cy5(Donkey polyclonal) | Jackson Immunoresearch | Cat #706-175-148RRID:AB_2340462 | (1:200) |
| Antibody | Anti-rRNA (mouse monoclonal Y10b) | Thermo Fisher ScientificLerner et al., 1981 | Cat #MA1-13017RRID:AB_10979967 | (1:100) |
| Antibody | anti-dRpS12 (guinea-pig polyclonal) | Kale et al., 2018 | (1:100) | |
| Antibody | rabbit anti-hRpL10Ab(rabbit polyclonal) | Sigma-Aldrich | Cat #SAB1101199; RRID: AB_10620774 | (1:200) |
| Antibody | anti-Rack1(rabbit monoclonal) | Cell Signalling Technology | Cat #D59D5RRID:AB_10705522 | (1:100) |
| Antibody | anti-RpS9(rabbit monoclonal) | Abcam | Cat #ab117861 RRID:AB_10933850 | (1:100) |
| Antibody | Anti-RpL9(rabbit monoclonal) | Abcam | Cat #ab50384RRID:AB_882391 | (1:100) |
| commercial assay, kit | Maxiscript T7 Transcription kit | Ambion | Cat #AM1312 | |
| other | ULTRAhyb-Oligo buffer | Ambion | Cat #AM8663 | |
| commercial assay, kit | Click-iT Plus OPP Alexa Fluor 594 or 488 Protein Synthesis Assay Kit | Thermo Fisher Scientific | Cat #C10457 | |
| Chemical compound, drug | Biotin-16-UTP | Roche | Cat #11388908910 | |
| Chemical compound, drug | RNA Sample Loading Buffer | Sigma-Aldrich | Cat #R4268-5VL | |
| Chemical compound, drug | Heat inactivated Fetal Bovine Serum | Gibco | Cat #10082139 | |
| Chemical compound, drug | Schneider’s Drosophila Medium | Gibco | Cat #21720024 | |
| Chemical compound, drug | Trizol | Ambion | Cat #15596–026 | |
| Chemical compound, drug | Odyssey Blocking buffer (PBS) | Li-COR | Cat #927–40003 | |
| sequence-based reagent | 18 S probe_Forward | Lee et al., 2018 | Invitrogen | GGTGCTGAAGCTTATGTAGC |
| sequence-based reagent | 18 S probe_Reverse | Lee et al., 2018 | Invitrogen | TAATACGACTCACTATAGGGAGACAAAGGGCA GGGACG |
| sequence-based reagent | 5.8 S probe_Forward | Lee et al., 2018 | Invitrogen | GCTTATATGAAACTAAGACATTTCG |
| sequence-based reagent | 5.8 S probe_Reverse | Lee et al., 2018 | Invitrogen | TAATACGACTCACTATAGGGTACATAAC AGCAT GGACTGC |
| sequence-based reagent | ITS2 probe_Forward | This study | Invitrogen | 5’- CTTTAATTAATTTTATAGTGCTGCTTGG-3’ |
| sequence-based reagent | ITS2 probe_reverse | This study | Invitrogen | 5’- TAATACGACTCACTATAGGGTTGT ATATAACTTTATCTTG-3’ |
| sequence-based reagent | 28 S probe_Forward | This study | Invitrogen | 5’-GCAGAGAGATATGGTAGATGGGC –3’ |
| sequence-based reagent | 28 S probe_reverse | This study | Invitrogen | 5’- TAATACGACTCACTATAGGGTTCCAC AATTGGCTACGTAACT-3’ |
| sequence-based reagent | ITS1 probe_Forward | This study | Invitrogen | 5’- GGAAGGATCATTATTGTATAATATC-3’ |
| sequence-based reagent | ITS1 probe_Reverse | This study | Invitrogen | 5’- TAATACGACTCACTATAGGGATG ATTACCACACATTCG-3’ |
| sequence-based reagent | 7SL probe_Forward | This study | Invitrogen | 5’- TCGACTGGAAGGTTGGCAGCTTCTG-3’ |
| sequence-based reagent | 7SL probe_Reverse | This study | Invitrogen | 5’- TAATACGACTCACTATAGGGATTGTGG TCCAACCATATCG-3’ |
| Other | VECTASHIELD antifade mounting medium | Vector Laboratories | Cat #H-1000 | |
| Other | Nuclear Mask reagent | Thermo Fisher Scientific | Cat #H10325 | |
| Cell line | S2-DGRC | Drosophila Genomics Resource Center (NIH Grant 2P40OD010949) | FLYBASE: FBtc0000006 RRID:CVCL_TZ72 | Stock #6(D. melanogaster embryonic cell line) |
| Other | Dual-Luciferase Reporter Assay System | Promega | Cat #E1910 | |
| Other | TransIT-2020 Transfection Reagent | Mirus Bio | Cat #MIR 5404 | |
| sequence-based reagent | Xrp target 1+ strand | This study | TCGAGATTGCACAACGCTCATTGCAC AACGTTCATTGCACAACGGCAATTGCACAACG |
|
| sequence-based reagent | Xrp target 1 - strand | This study | TCGACGTTGTGCAATTGCCGTTGTGCAATG AACGTTGTGCAATGAGCGTTGTGCAATC |
|
| sequence-based reagent | Xrp target 2+ strand | This study | TCGAGCATGATGAAATAACATGCTCCATGA TGAAATAACATGTTCCATGATGAAATAACA TGGCACATGATGAAATAACATG |
|
| sequence-based reagent | Xrp target 2 - strand | This study | TCGACATGTTATTTCATCATGTGCCATGTTA TTTCATCATGGAACATGTTATTTCATCATG GAGCATGTTATTTCATCATGC |
|
| sequence-based reagent | Xrp target 3+ strand | This study | TCGAGATTACATCATGCTCATTACATC ATGTTCATTACATCATGGCAATTACATCATG |
|
| sequence-based reagent | Xrp target 3 - strand | This study | TCGACATGATGTAATTGCCATGATGTAATG AACATGATGTAATGAGCATGATGTAATC |
|
| sequence-based reagent | Xrp trgt 1+ strand shuffled | This study | TCGAGTGACAACTCAGCTCTGACAACTCAGT TCTGACAACTCAGGCATGACAACTCAG |
|
| sequence-based reagent | Xrp trgt 1 - strand shuffled | This study | TCGACTGAGTTGTCATGCCTGAGTTGTCAGAA CTGAGTTGTCAGAGCTGAGTTGTCAC |
|
| sequence-based reagent | Xrp trgt 2+ strand shuffled | This study | TCGAGTTCAAATCAATAGGAAGCTCTTCAAATCA ATAGGAAGTTCTTCAAATCAATAGGAAGGCATTC AAATCAATAGGAAG |
|
| sequence-based reagent | Xrp trgt 2 - strand shuffled | This study | TCGACTTCCTATTGATTTGAATGCCTTCCTATT GATTTGAAGAACTTCCTATTGATTTGAAGAGC TTCCTATTGATTTGAAC |
|
| recombinant DNA reagent | pGL3-Promoter Vector | Promega | Cat #E1761 | |
| recombinant DNA reagent | pAct5.1/V5-His C vector | Thermo Fischer Scientific | Cat #V411020 | |
| recombinant DNA reagent | pIS1 plasmid | Addgene | Cat #12,179 | |
| recombinant DNA reagent | pUAST vector | Brand and Perrimon, 1993 | FLYBASE:FBmc0000383 | Drosophila Genomics Resource Center#1,000 |
| recombinant DNA reagent | pGL3-Rluc | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | p-GL3-H-T1 | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | p-GL3-H-T2 | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | p-GL3-H-T3 | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | p-GL3-H-T1S | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | p-GL3-H-T2S | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | pGL3-X-T1 | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | pGL3-X-T2 | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | pGL3-X-T3 | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | pGL3-X-T1S | This study | See Materials and Methods; Dr. Nicholas Baker’s lab | |
| recombinant DNA reagent | pGL3-X-T2S | This study | See Materials and Methods; Dr. Nicholas Baker’s lab |