Figure 5.

THC inhibits iNOS, COX-2, and p-NFκB expressions in P. a. LPS-stimulated BV2 microglial cells. (a) BV2 microglial cells were pretreated without (mock) or with THC (10, 20, or 40 μM) for 1.5 h following stimulation with P. a. LPS (0.1 μg/ml) for 24 h. Western blotting showed the protein expression of iNOS, COX-2, p-NFκB, and GAPDH. Statistical results of (b) iNOS, (c) COX-2, and (d) p-NFκB p50 were shown. The experimental quantitative data are presented as means ± SD (n = 3). Bars with the same letter represent no significant difference between the groups. Bars with different letters indicate a statistically significant (p < 0.05) difference between the groups.