Figure 6.

THC reverses the inhibition of HO-1 inhibitor Snpp in P. a. LPS–stimulated BV2 microglial cells. (a) BV2 microglial cells were untreated (the mock group) or pretreated with THC (10, 20, or 40 μM) for 1.5 h before being stimulated with P. a. LPS (0.1 μg/ml) for 24 h. Western blotting was used to obtain the expressions of p-Nrf2, HO-1, and GAPDH proteins. (b) BV2 microglial cells were pretreated with THC (40 μM) or HO-1 inhibitor Snpp (20 μM) for 1.5 h before being stimulated with P. a. LPS (0.1 μg/ml) for 24 h. Western blotting was used to obtain the expressions of p-Nrf2, HO-1, and GAPDH proteins. Statistical results of (c) p-Nrf2 and (d) HO-1 were shown. The experimental quantitative data are presented in terms of the mean ± SD (n = 3). Bars with the same letter represent no significant difference between the groups. Bars with different letters indicate statistical significance (p < 0.05) between the groups.