Skip to main content
. 2022 Mar 11;2022:4564471. doi: 10.1155/2022/4564471

Figure 3.

Figure 3

Omav reduced the expression of inflammatory cytokines but enhanced BV2 phagocytosis under the stress of OxyHb. (a–c) Levels of the Il-1β, Tnf-α, and Il-10 mRNAs in treated BV2 cells (n = 3). (d) Representative fluorescent images of the phagocytosis assay in BV2 cells. β-Actin in BV2 cells was labeled with phalloidin-FITC, and RBCs were labeled with DiI. Scale bar: 50 μm. (e) Quantification of the phagocyte proportion according to fluorescence (n = 6). (f and g) The concentrations of IL-1β and TNF-α were detected using ELISAs (n = 6). (h) Phagocytosis ratio of BV2 cells in each group detected via FCM. (i) Quantification of the ratio of BV2 cells phagocytoses labeled RBCs from each group (n = 3). Data are presented as the means ± SEM. ∗P < 0.05 and ∗∗P < 0.01 compared with the control group; #P < 0.05 and ##P < 0.01 compared with the OxyHb group; †P < 0.05 and ††P < 0.01 compared with the OxyHb+Omav group.