TABLE 1.

Probe melting temperatures and nucleotide sequence results for codon 550

Patient (genotype) and mos of treatment	Mean probe melting temperature (°C) ± SDa			Temperature change relative to pretreatment (°C)			Interpretation	Sequence result
	WT (ATG)	M1 (GTG)	M2 (ATT)	WT (ATG)	M1 (GTG)	M2 (ATT)		Population	Cloning
A (D)
0	62.2 ± 0.8	59.5 ± 0.4	54.3 ± 0.4				ATG	ATG
9	63.2 ± 0.9	60.4 ± 0.6	54.3 ± 0.4	<1	<1	<1	ATG	ATG
23	56.5 ± 0.2	55.6 ± 1.0	60.9 ± 0.1	−5.7	−3.9	6.6	ATT	ATT	ATT (15 clones)
B (C)
0	62.0 ± 0.4	60.4 ± 0.6	54.1 ± 0.6				ATG	ATG
25	57.0 ± 0.7	54.5 ± 0.6,	52.0 ± 1.0,	−5.0	−5.9	−2.1	GTG & ATT	(A/G)T(G/T)	8 GTG 12 ATT
		64.1 ± 0.4b	60.6 ± 0.7b		3.7	6.5
31	56.7 ± 0.4	53.8 ± 0.6,	51.8 ± 0.4,	−5.3	−6.6	−2.3	GTG & ATT	(A/G)T(G/T)
		65.0 ± 1.1b	602. ± 0.3b		4.6	6.1
C (B)
0	61.3 ± 0.6	58.6 ± 0.4	53.6 ± 0.3				ATG	ATG
19	55.7 ± 0.3	54.8 ± 1.1	59.4 ± 0.6	−5.6	−3.8	5.8	ATT	ATT
D (D)
0	61.8 ± 0.3	59.0 ± 0.7	54.7 ± 0.6				ATG	ATG
29	56.5 ± 0.3	55.3 ± 0.1	61.0 ± 1.0	−5.3	−3.7	6.3	ATT	ATT
E (D)
0	61.7 ± 0.3	59.3 ± 0.4	54.4 ± 0.6				ATG	ATG
28	57.0 ± 0.2	56.6 ± 0.2	59.8 ± 0.6	−4.7	−2.7	5.4	ATT	ATT
F (A)
0	61.6 ± 0.1	60.2 ± 0.4	54.1 ± 0.7				ATG	ATG
16	55.7 ± 0.5	54.1 ± 0.7	60.5 ± 0.9	−5.9	−6.1	6.4	ATT	ATT
G (A)
0	60.4 ± 1.0	59.3 ± 1.1	54.1 ± 0.6				ATG	ATG
13	57.3 ± 0.7	62.4 ± 0.8	51.1 ± 0.5	−3.1	3.1	−3.0	GTG	GTG
Off 3c	56.2 ± 1.6	No clear peak	No clear peak	−4.2	?	?	Not ATG	(A/G)T(C/G)	4 ATC 10 GTG
H (D)
2	62.4 ± 1.0	60.6 ± 0.3	54.7 ± 0.9				ATG	ATG
12	57.5 ± 0.5	63.6 ± 0.5	51.6 ± 0.5	−4.9	3.0	−3.1	GTG	GTG
Off 6c	62.0 ± 0.3	60.1 ± 0.5	53.9 ± 0.8	<1	<1	<1	ATG	ATG

^aMeans were derived from at least three determinations. 

^bTwo peaks. 

^cOff treatment.