Fig. 1. Lack of Fdxr, Trp53, or both leads to altered lipid metabolism though the ABCA1–SREBP pathway in MEFs.
A The levels of ABCA1, SREBP1/2, MVD, MVK, and actin were measured in WT, Fdxr+/−, Trp53+/−, and Fdxr+/−;Trp53+/− MEFs cultured in serum-free media for 4 h. B WT, Fdxr+/−, Trp53+/−, and Fdxr+/−;Trp53+/− MEFs were cultured in serum-free media for 8 h and then were stained with Nile Red (ex: 488 nm, em: 565 nm). DAPI (ex: 358 nm, em: 461 nm) was used to stain nuclei. C Quantitative measurement of intracellular cholesterol. WT, Fdxr+/−, Trp53+/−, and Fdxr+/−;Trp53+/− MEFs were cultured in a 96-well plate. After 4 h of fasting, the level of total cholesterol was measured with Cholesterol/Cholesterol Ester-GloTM assay kit according to the manufacturer’s instruction. Data represent the mean ± SD. D Quantitative measurement of intracellular triglyceride. WT, Fdxr+/−, Trp53+/−, and Fdxr+/−;Trp53+/− MEFs were cultured in a 96-well plate. After 4 h of fasting, the level of total triglycerides was measured with Triglyceride-GloTM assay kit according to the manufacturer’s instruction. Data represent the mean ± SD.