Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 31;41(12):1679–1690. doi: 10.1038/s41388-022-02199-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Expression of hnRNP E1 and the mesenchymal markers, N-cadherin and Vimentin, in NMuMG cells treated with TGFβ. Hsp90 was used as a loading control. b Schematic of ARIH1-miniTurboID pulldown strategy (top panel). Immunoblot of biotin and hnRNP E1 in Hek293 cells either mock transfected or transfected with ARIH1-miniTurboID and treated with or without Biotin for 3 h. c Stable KD of ARIH1 by shRNA (top panel) and hnRNP E1 protein stability in control and ARIH1 KD NMuMG cells (bottom panel). d Ubiquitination of exogenous V5 tagged hnRNP E1 detected by V5 immunoprecipitation and K48 ubiquitin immunoblot. e Protein stability, assessed by cycloheximide chase assay, of WT and K314R or K351R mutant V5-tagged hnRNP E1. f Ubiquitination of WT and K314R V5 tagged hnRNP E1 detected by V5 immunoprecipitation and immunoblot.