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. 2022 Feb 9;41(12):1821–1834. doi: 10.1038/s41388-022-02212-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Cellular extracts from SK-Hep1 and HLF cells transfected with FLAG-tagged DDR1 or FLAG-tagged vector were subsequently immunoprecipitated with anti-FLAG antibody. Eluted proteins were separated on SDS-PAGE and visualized by Coomassie blue staining. Eluted proteins were identified by mass spectrometry analysis. B Selective genes from mass spectrometry analysis were co-immunoprecipitated with Myc-tagged DDR1 (up). Detection of ARF6 by mass spectrometry (bottom). C 293 T cells were transiently co-transfected with indicated plasmids and co-immunoprecipitation assays were performed. D Immunoblots of co-immunoprecipitated (IP) endogenous DDR1 and endogenous ARF6 in HLE cell extracts. Immunoglobulin G (IgG) is negative control. E Confocal assays were shown to observe the co-localization of exogenously expressed DDR1 and ARF6 in 293 T cells (Scale bar: 15 μm). F Confocal assays were shown to observe the co-localization of endogenous DDR1 and ARF6 in HLF and HLE cells (Scale bar: 30 μm). G Schematic diagram of FLAG-tagged full-length or deletion constructs of ARF6 used in this study (left panel). Co-precipitation of HA-tagged DDR1 with FLAG-tagged ARF6 or its mutants, analyzed by anti-FLAG immunoprecipitation and anti-FLAG/ HA immunoblots (right panel).