Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 5;41(12):1752–1766. doi: 10.1038/s41388-022-02203-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A Shown is the strategy for the generation of EGLN3R205K knock-in (KI) mouse strain using CRISPR/Cas9 approach. B Genotyping of tail genomic DNA from wild-type or KI mouse by conventional PCR (n = 3). C DNA sequencing of genomic DNA from KI mouse (n = 2). D, F RT-PCR analysis of EGLN3 and R205K mRNA levels in the kidney (D) and heart (F) from wild-type or KI mouse (n = 3). E, G Immunoblotting analysis of EGLN3 and R205K protein levels in the kidney (E) and heart (G) from wild-type or KI mouse (n = 3). H, I Immunohistochemistry analysis of EGLN3 and R205K expression in the kidney (H) and heart (I) from wild-type or KI mouse (n = 3). J Immunohistochemistry analysis of Erk3 levels in the heart of wild-type or KI mouse (n = 3). K–M Immunoblotting analysis of Erk3 expression in the heart (K), aorta (L) and bone marrow-derived macrophages (BMDM) (M) of wild-type or KI mouse. GAPDH and Tubulin were used as the loading control (n = 3). CRISPR clustered regularly interspaced short palindromic repeats; ssODNs single-stranded DNA oligonucleotides; PAM protospacer adjacent motif; gRNA guide RNA; WT wild-type; KI knock-in; BMDM bone marrow-derived macrophages.