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. 2022 Feb 5;41(12):1752–1766. doi: 10.1038/s41388-022-02203-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A GST pulldown analysis of the effect of EGLN2, EGLN3, or R205K on the interaction between Erk3 and HSC70 (n = 3). B GST pulldown analysis of the effect of EGLN1, EGLN2, EGLN3, or R205K on the interaction between Erk3 and LAMP2A (n = 3). C GST pulldown analysis of the impact of EGLN3 and R205K on the interaction between Erk3 and LAMP2A (n = 3). D GST pulldown analysis of the impact of EGLN3 activity on the interaction between Erk3 and LAMP2A (n = 4). E, F GST pulldown analysis of the effect of EGLN3 on Erk3(341–721) interaction with LAMP2A (E) and HSC70 (F) (n = 3). G, H IB analysis of the influence of EGLN3 and R205K on Erk3 degradation induced by HSC70 or LAMP2A (n = 3). I IB analysis of the effect of DMOG on Erk3 interaction with HSC70 or LAMP2A (n = 3). J, K GST pulldown analysis of Erk3 or hydroxylation-resistant mutant Erk3(P25A) interaction with LAMP2A (J) and HSC70 (K) (n = 3). L Cycloheximide chase experiment was conducted to measure the stability of Erk3 or its mutant Erk3(P25A) (n = 3). M GST pulldown analysis of Erk3 or hydroxylation-resistant mutant Erk3(P25A) interaction with EGLN3 or R205K (n = 3). N, O IB analysis was done to examine the expression of relative proteins in HEK293T cells transfected the indicated constructs (n = 3). Tub tubulin; HSC70 heat shock cognate protein of 70 kDa; LAMP2A lysosome-associated membrane protein type 2A; PD pull down; GST glutathione S-transferase; IB immunoblotting; DMOG dimethyl oxalyglycine; CHX cycloheximide.