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. 2022 Feb 5;41(12):1752–1766. doi: 10.1038/s41388-022-02203-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A, B Wound assay was conducted to determine the role for Erk3 in macrophage migration. BMDM were transfected with control or Erk3 siRNA for 48 h and then scratched. The cells in the denuded regions were counted and statistically analyzed. C IB analysis of the expression of proteins indicated in BMDM treated with control (−) or Erk3 (+) siRNA. D, E IB analysis of NRP1 expression in macrophages infected with lentivirus expressing Erk3 (D) or HEK293T or Hep3B cells transfected with plasmid expressing Flag-Erk3 (E). F, G Wound assay to evaluate the role for EGLN3 inactivation in macrophage migration. H, I in vivo migration of macrophages. Peritoneal macrophages in the lavages were collected from WT and KI mice injected intraperitoneally with thioglycollate, stained with PE-conjugated anti–F4/80 and quantified by flow cytometry. J qPCR analysis of NRP1 mRNA expression in WT and KI BMDM. K, L IB analysis of NRP1 and NRP2 expression in WT and KI BMDM. M Efferocytosis assay was performed to evaluate the ability of WT and KI BMDM to take up apoptotic Jurkat T cells labeled with Calcein AM. Efferocytosis was quantitated. N, O The expression of Arg1 mRNA (N) and protein (O) in WT and KI BMDM treated with (+) or without (−) IL-4 were analyzed by qPCR and IB analysis, respectively. P IB analysis of iNOS expression in WT and KI BMDM treated with (+) or without (−) LPS and IFNγ. Q Wound assay was pursued to evaluate the effect of DMOG on macrophage migration. R, S IB analysis of NRP1 and NRP2 expression in DMOG-treated BMDM. BMDM bone marrow-derived macrophages; siRNA small interfering RNA; NRP neuropilin; IB immunoblotting; DMOG dimethyl oxalyglycine; WT wild-type; KI knock-in; Arg1 arginase 1; iNOS inducible nitric oxide synthase; IL-4 interleukin 4; LPS lipopolysaccride; IFNγ interferon γ; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by unpaired 2-tailed Student’s t test. Shown are representative results of three independent experiments.