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. 2022 Mar 18;13:1478. doi: 10.1038/s41467-022-29125-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Motif sequence at m⁷G site. b Representative image of cleavage score. c Quantification of m⁷G level on m⁷G-modified tRNAs. d LC-MS-based detection of m⁷G tRNA modification levels on tRNAs. e Expression profile of m⁷G-modified tRNAs in the METTL1 knockdown and control cells. Each cell shows the relative expression of all isodecoders of a specific tRNA type. Expression of each tRNA type was normalized by its overall average level in both groups and transformed by log2. f Mann–Whitney U test on the expression of the m⁷G-modified and non-m⁷G tRNAs. g–j Northern blot of indicated tRNAs. U6 snRNA was used as a loading control. k Polysome profiling of METTL1 depleted and control K150 cells. l Puromycin intake assay of K150 cells. m Schematic of polyribosome-seq. n The numbers of m⁷G tRNA-decoded codons in mRNAs with upregulated TEs (up), downregulated TEs (down), and unaltered TEs (non) in the METTL1 depleted cells. o Gene ontology analysis using the TE-down genes. p Pathway analysis using the TE-down genes. q m⁷G tRNA-decoded codon numbers in TE-decreased mRNAs enriched in the negative regulation of autophagy or mTOR signaling pathway and other mRNAs. r qRT-PCR analysis of representative genes in K150 cells. s Western blot analysis of indicated proteins in K150 cells, right panel: quantification of the western blot signals. t qRT-PCR based TE analysis of RPTOR using the polyribosome mRNAs. u Western blot analysis of ULK1 and pULK1 in K150 cells, right panel: quantification of the western blot signals. v Western blot analysis of LC3 in K150 cells, right panel: quantification of the western blot signals. Data presented as mean ± SD from two independent biological samples for each group for (c, e, and f), three independent biological samples for (d), one biological sample for (n, q), and three independent experiments for (r–v). P values are calculated by Mann–Whitney U test for (c, f, n, and q), and two-tailed unpaired Student’s t test for (d, r, s, t, u, and v). Source data are provided as a Source data file.