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. 2022 Mar 18;13:1478. doi: 10.1038/s41467-022-29125-7

Fig. 4. METTL1/RPTOR/ULK1 axis plays essential roles in ESCC progression.

Fig. 4

a Western blot analysis with indicated antibodies in METTL1 knockdown K150 cells with or without RPTOR overexpression. b CCK8 assay of METTL1 knockdown K150 cells with or without RPTOR overexpression. c, d Colony-formation assay (c) and the quantification analysis (d) of METTL1 knockdown K150 cells with or without RPTOR overexpression. e Western blot analysis with indicated antibodies in METTL1 knockdown K150 cells with or without RPTOR overexpression. f–h The autophagic fluxes (f) and the quantification analysis (g, h) of K150 cells stably expressed mRFP-EGFP-LC3 fusion protein. Scale bar, 10 μm. i Western blot analysis with indicated antibodies. j CCK8 assay of METTL1 knockdown K150 cells with or without ULK1 knockdown. k–m The autophagic fluxes (k) and the quantification analysis (l, m) of K150 cells stably expressed mRFP-EGFP-LC3 fusion protein. Scale bar, 10 μm. Data represented as mean ± SD from three independent experiments. P values are presented by one-way ANOVA with Tukey’s multiple comparison test for (b, d), and one-way ANOVA with Dunnett’s multiple comparison test for (g, j–h, and l, m).