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. 2022 Mar 18;13:1478. doi: 10.1038/s41467-022-29125-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a The catalytic mutation site of METTL1. b Western blot confirmed the overexpression of wild-type METTL1 (oeWT) and catalytic inactive METTL1 (oeMUT) compared with the negative control (oeNC) in ESCC cells. c CCK8 assay of growth of oeNC, oeWT, and oeMUT ESCC cells. d, e Colony-formation assay (d) and the quantification analysis (e) of oeNC, oeWT, and oeMUT ESCC cells. f qRT-PCR assay of RPTOR mRNA level. g Western blot assay showed the indicated protein levels. h, i Apoptosis assay (h) and the quantitative analysis (i) of METTL1 overexpression and control cells. j Western blot assay showed the indicated protein levels. k CCK8 assay of growth of oeNC and oeWDR4 ESCC cells. l, m Colony-formation assay (l) and the quantification analysis (m) of oeNC and oeWDR4 ESCC cells. Data represented as mean ± SD from three independent experiments. P values are presented by one-way ANOVA with Dunnett’s multiple comparison test for (c, e, f, and i), and two-tailed unpaired Student’s t test for (k and m).