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. 2022 Mar 18;12:4718. doi: 10.1038/s41598-022-08759-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular apoptosis. (a) Flow cytometry histograms of A-549 cells treated with Free DOX, PLA-PEG NPs, PLA-PEG/DOX NPs, PLA-PEG-Apt NPs, and PLA-PEG-Apt/DOX NPs for 24 h using Annexin V-FITC/PI staining (Q1: dead cells with Annexin V-FITC (−)/PI (+), Q2: late apoptotic cells with Annexin V-FITC (+)/PI (+) Q3: live cells with AnnexinV-FITC (−)/PI (−), Q4: early apoptotic cells with Annexin V-FITC (+)/PI (−)). (b) The number of apoptosis cells was counted with a FACS Calibur flow cytometer for A-549 cells (P-value: ns is not-significance (≥ 0.05). * is significant between 0.01 and 0.05., ** is significant between 0.001 and 0.01., and ****, significant < 0.0001).