Fig. 6. Direct and abscopal effects of GSNO and aCTLA-4 loaded F127-g-Gelatin hydrogel.

a Tumor model and treatment schedule. 1^o and 2^o tumors were formed in C57Bl/6 mice by inoculation of 10⁵ B16F10-OVA cells in 30 μL saline on day 0 and day 4, respectively. GSNO (570 μg kg⁻¹) and aCTLA-4 (50 μg mouse⁻¹) were administered intratumorally on day 7 in a total volume of 30 μL in saline or 4.5 wt.% F127-g-Gelatin hydrogel. Blood was harvested from the facial vein on day 9 for assessment using the ALT/AST assay (n = 4 for HG and aCTLA-4/HG, and n = 5 for the other). b Relative body weight changes post treatment (n = 5). c ALT/AST activities of blood taken from mice 2 d after treatment (n = 5). d Kaplan–Meier survival curves (n = 5). e 1^o (directly injected) tumor size (n = 5). f 2^o (uninjected) tumor size (n = 5). Data are presented as individual biological replicates and mean ± SEM. *****p < 0.0001, ****p < 0.001, ***p < 0.01, **p < 0.05, and *p < 0.1. Exact p-values for d–f are reported in Supplementary Tables 8 and 9. b Two-way ANOVA using Tukey post-hoc statistical hypothesis. c One-way ANOVA using Tukey post-hoc statistical hypothesis. d Log-rank using Mantel–Cox statistical hypothesis. e, f ANOVA using linear mixed-effects model. Source data are available in a Source Data file.