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. 2022 Mar 18;13:1479. doi: 10.1038/s41467-022-29121-x

Fig. 7. Antitumor effects of GSNO and aCTLA-4 loaded F127-g-Gelatin hydrogel on 4T1 tumor models.

Fig. 7

a Tumor model and treatment schedule. 1o and 2o tumors were formed in Balb/C mice by inoculation of 3 × 105 4T1 cells in 30 μL saline to left mammary fat pad on day 0 and at right mammary fat pad on day 4, respectively. GSNO (570 μg kg−1) and aCTLA-4 (50 μg mouse−1) were administered intratumorally on day 7 in a total volume of 30 μL in saline or 4.5 wt.% F127-g-Gelatin hydrogel. b Relative body weight changes post treatment. c Kaplan–Meier survival curves. d 1o (directly injected) tumor size. e 2o (uninjected) tumor size. Data are presented as individual biological replicates and mean ± SEM. n = 11 for saline, HG, and Free GSNO + aCTLA-4. n = 12 for GSNO + aCTLA-4/HG. *****p < 0.0001, ****p < 0.001, ***p < 0.01, **p < 0.05, and *p < 0.1. Exact p-values for c–e are reported in Supplementary Tables 11 and 12. b Two-way ANOVA using Tukey post-hoc statistical hypothesis. c Log-rank using Mantel–Cox statistical hypothesis. d, e ANOVA using linear mixed-effects model. Source data are available in a Source Data file.