Figure 1.
Cell viability of PBMCs in the presence of Convulxin (CVX). Human PBMCs isolated from leukocytes from healthy adult blood donors by a density gradient method viability in the absence or presence of inhibitors was measured by MTT metabolization method (A). Isolated 2 × 105 PBMCs were incubated with RPMI (control) or with different concentrations of CVX (0.3 to 20 μg/mL; experimental group) for 1 h (B), or CVX (5 and 10 μg/mL; experimental group) for 12 (C), 24 (D), 48 (E), 72 (F), and 96 h (G). Viability was then assessed by PI and TO method in FACScan. The results were expressed in % and represent the mean ± S.E.M. from 4 to 5 donors.