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. 2022 Mar 18;13:1449. doi: 10.1038/s41467-022-28938-w

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Fig. 6 — Assays for the determination of Michaelis constants were carried out in duplicate at 5 °C intervals from 20 to 40 °C from a single batch of purified enzyme as described in the “Methods” section. Errors represent the standard error of the mean for k_cat values determined by non-linear regression.