Table 2.

Kinetic parameters of OtCE15A variants from this (marked with *) and previous work^9,10.

Enzyme	Km (mM)	kcat (s−1)	kcat/Km (s−1 M−1)
BnzGlcA
Wild Type	3.57 ± 0.091	16.6 ± 0.110	4.65E+03 ± 1.2E+02
S267A	2.83 ± 0.660	0.000983 ± 0.000059	3.47E−01 ± 8.3E−02
H408A	0.184 ± 0.035	0.00999 ± 0.000400	5.43E+01 ± 1.1E+01
E290A	2.03 ± 0.110	10.3 ± 0.110	5.07E+03 ± 2.8E+02
D356A	1.86 ± 0.110	5.21 ± 0.073	2.80E+03 ± 1.7E+02
E290A/D356A	0.502 ± 0.034	0.196 ± 0.003	3.90E+02 ± 2.7E+01
R268A*	0.408 ± 0.051	0.204 ± 0.007	5.01E+02 ± 6.5E+01
MeGlcA
Wild Type*	2.77 ± 0.150	19.0 ± 0.310	6.85E+03 ± 3.9E+02
R268A*	0.386 ± 0.060	0.214 ± 0.009	5.54E+02 ± 9.3E+01

The error on the kinetic parameters was calculated by non-linear regression based on duplicate measurements at 8 substrate concentrations.