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. 2022 Mar 18;12:4681. doi: 10.1038/s41598-022-07887-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Optimization experimental design. Based on (A) the silicone-CaO₂ O₂ production rate and (B) the MIN6 pseudo-islet O₂ consumption rate, (C) a central composite design was used to optimize the HEMOXCell concentration and islet seeding density in the BAP. The response variables, intracellular ATP content (RLU), and ATP/LDH viability ratio (RLU/AU) per device were assessed for MPIs encapsulated in alginate sheets after 1 day of culture. Results of O₂ production rate (n = 2) and consumption rate (n = 6) are presented as mean ± SEM of independent experiments. (D) Response surface analysis for the optimization DoE. The plots represent the effects of the HEMOXCell concentration, islet seeding density, and their interaction on the ATP content and the ATP/LDH ratio in the BAP after 1 day of culture under 1% O₂ tension.