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. 2022 Mar 18;12:4681. doi: 10.1038/s41598-022-07887-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Viability and function of MIN6 pseudo-islets (MPIs) embarked in O₂-balanced BAP. Alginate encapsulated MPIs (3000 IEQ/150 µL) were cultured for 3 days under 20% O₂ (positive control, white) or 1% O₂ condition without O₂ strategy (negative control, black) or with innovative strategy of oxygenation (ISO) composed of silicone-CaO₂ disk and 500 µg/mL HEMOXCell by alginate (1% O₂ + ISO, grey). (A) Total metabolic activity (ATP content, RLU), (B) viability (ATP/LDH ratio, RLU/AU), (C) Glucose plus theophylline (G + T) responsive insulin release by 30 min sequential incubations of alginate encapsulated MPIs in basal medium (black bar) and glucose plus theophylline stimulation (grey bar), (D) Stimulation index (ratio of high glucose plus theophylline stimulation over basal insulin secretion). Results from independent experiments (n = 5–9) are expressed as mean ± SEM. *p < 0.05, **p < 0.01 (unpaired parametric t-test).